Journal: Biomolecules
Article Title: Effects of GHR Deficiency and Juvenile Hypoglycemia on Immune Cells of a Porcine Model for Laron Syndrome
doi: 10.3390/biom13040597
Figure Lengend Snippet: Lymphocyte populations, lymphocyte subpopulations and proliferative capacities of PBMC of WT and GHR -KO pigs. ( A ) Percentage of lymphocytes in the leukocytes of wild-type (WT) (black dots, n = 7) and growth hormone receptor knockout ( GHR -KO) pigs (blue dots, n = 6). Lymphocyte percentages were not significantly different between WT and GHR -KO pigs. ( B – I ) Lymphocyte subpopulations of WT (black dots, n = 16) and GHR -KO pigs (blue dots, n = 15). Proportions of ( B ) CD79a + B cells, ( C ) CD3 + T cells ( D ) CD8α + CD3 − NK cells, ( E ) CD8α + CD3 + T cells, ( F ) SWC5 + γδ T cells, ( G ) CD4 − CD8α + lymphocytes, ( H ) CD4 + CD8α + activated/memory T cells did not significantly differ between WT and GHR -KO pigs. CD4 + CD8α − cells ( I ) significantly differed between WT and GHR -KO pigs. ( J – M ) Proliferative capacity of peripheral blood mononuclear cells (PBMCs) of WT (black dots, n = 10) and GHR -KO pigs (blue dots, n = 10) after polyclonal stimulation with ( J ) pokeweed mitogen (PWM), ( K ) concanavalin A (ConA), ( L ) phytohaemagglutinin-L (PHA-L) and ( M ) M. paradisiaca lectin (BanLec) in vitro revealed similar proliferative capacity of WT and GHR -KO PBMCs. Data are shown as mean ± SD; ns = not significant, * p ≤ 0.05.
Article Snippet: We used FITC-conjugated mouse anti-pig CD4α mab (clone MIL17, IgG2b; Bio-Rad AbD Serotec; 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α mab (clone 76-2-11, IgG2a; Becton Dickinson, Heidelberg, Germany; 1:200) to identify αβ T cells, and Alexa Fluor 647-conjugated mouse anti-pig SWC5 mab (clone b37c10, IgG1; Bio-Rad AbD Serotec; 1:5) for the identification of a γδ T cell subpopulation (WT: n = 6; GHR -KO: n = 5).
Techniques: Knock-Out, In Vitro
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